Table of Contents
- 1 What do you use to adjust the amount of light shining on the specimen?
- 2 What do you do if you need more light to view your specimen?
- 3 How can the amount of light illuminating the specimen be controlled?
- 4 Why must a specimen be centered?
- 5 Is used to collect the light and direct it to the specimen?
- 6 Which part is responsible for the supply of light in a compound microscope?
- 7 How do you adjust a microscope to see clearly?
- 8 What do you need to know about specimen requirements?
- 9 Do you need a cover slip for a high magnification microscope?
What do you use to adjust the amount of light shining on the specimen?
IRIS DIAPHRAGM CONTROL — A lever (or rotating disk) that adjusts the amount of light illuminating the slide. Use just enough light to illuminate the object on the slide and give good contrast.
What do you do if you need more light to view your specimen?
Always begin viewing a slide using the microscope’s lowest magnification. This reduces the risk of contact by the microscope’s objective lens. Afterwards, switch to a higher magnification if needed.
How can the amount of light illuminating the specimen be controlled?
The condenser is equipped with an iris diaphragm, a shutter controlled by a lever that is used to regulate the amount of light entering the lens system. Above the stage and attached to the arm of the microscope is the body tube. This structure houses the lens system that magnifies the specimen.
What provides light for viewing the specimen?
Diaphragm Controls the amount of light that passes through the specimen. Light source Provides light for viewing the specimen. Using the Compound Light Microscope: 1.
How do you adjust a light microscope?
The microscope rheostat control can be found on the side of the compound microscope body. It will typically be a knob that is turned clockwise in order to increase the light intensity, or counter-clockwise to reduce the light.
Why must a specimen be centered?
You must have the object centered before you change objectives to increase the magnification, because the field of view becomes smaller; if the object is off to the side, it may disappear when you go to higher magnification. For best viewing at high power, white light is essential.
Is used to collect the light and direct it to the specimen?
A microscope gets its light from the specimen in two ways. It’s used to direct room light, lamp light, or skylight from below the scope’s stage up through the specimen as transmitted light. Mirror illuminators most often have flat and concave sides. The flat side simply reflects light and gives a sharper image.
Which part is responsible for the supply of light in a compound microscope?
Iris Diaphragm controls the amount of light reaching the specimen. It is located above the condenser and below the stage. Most high quality microscopes include an Abbe condenser with an iris diaphragm.
Which is the correct path of light in a compound microscope?
The path of light through a microscope. Modern microscopes are complex precision instruments. Light, originating in the light source (1), is focused by the condensor (2) onto the specimin (3). The light then enters the objective lens (4) and the image is magnified.
Is light enters the eyepiece first?
The light then enters the objective lens (4) and the image is magnified. Light then passes through a series of glass prisms and mirrors, eventually entering the eyepiece (5) where is it further magnified, finally reacing the eye. First let us consider a primary feature of all microscopes, the light source.
How do you adjust a microscope to see clearly?
Turn the coarse focus knob slowly until you are able to see the cells. Turn the fine focus knob slowly until the cells are in focus and you can see them clearly.
What do you need to know about specimen requirements?
Specimen requirements include information such as specimen volume, collection and transport containers as well as transport temperature. If additional information is needed for the interpretation of the test results or there are specific instructions for patient preparation, they are listed along with specimen requirements.
Do you need a cover slip for a high magnification microscope?
The cover slip must be up if there is one. High magnification objective lenses can’t focus through a thick glass slide; they must be brought close to the specimen, which is why coverslips are so thin. The stage may be equipped with simple clips (less expensive microscopes), or with some type of slide holder.
How does a mirror illuminator get light to the specimen?
Mirror illuminators most often have flat and concave sides. The flat side simply reflects light and gives a sharper image. The concave side concentrates the light and provides brighter illumination. It can be difficult to direct the light with a mirror illuminator or it may not be bright enough to do the job.
What are the general guidelines for specimen handling?
GENERAL GUIDELINES. The requested volume is an amount sufficient to allow at least two performances of the assay either singly or in duplicate. The minimum volume allows one single analysis including instrument dead volume. Storage temperature is specified as room temperature (15 – 30°C), refrigerated (2 to 10°C) or frozen (-20°C or colder).