How do you sterilize agar plates?

How do you sterilize agar plates?

The most effective and suitable method of sterilising agar is by using moist heat in the form of steam under pressure i.e. 121oC for 15 minutes at 15 pounds per square inch (psi). This method will denature & coagulate enzymes and other cell constituents in the bacterial cell.

Are Petri dishes reusable?

Petri dishes are typically made of borosilicate glass or clear plastics (usually polystyrene or polycarbonate) and come in a variety of sizes. They can be disposable or reusable, with reusable types able to withstand repeated sterilization procedures (wet or dry).

Why did you Sterilise the Petri dishes?

Preparing the agar plates of a colony of bacteria Glass Petri dishes and agar gel must be sterilised before use in an autoclave , or pre-sterilised plastic Petri dishes can be bought. Reason – this will kill any bacteria that are present in the solution or on the Petri dishes.

What is the proper procedure for petri plate disposal?

You can do this with just regular bleach. Give it a swirl in the dishes, making sure it’s hitting all the surfaces, and dump it. Be careful not to get the bleach on your skin or eyes! Wear protection if necessary!

Can you microwave petri dishes?

They showed that 1-5 minutes in a conventional microwave was sufficient to decontaminate 5mL cultures or petri dishes of common clinical pathogens including E.

Can you PC plastic petri dishes?

No Pour Method There are many heat resistant containers that can withstand being pressure cooked, but disposable plastic petri dishes are not one of them. Surprisingly, these containers will withstand being pressure cooked.

How many hours should the Petri dishes be sterilized?

Sterilizing glass Petri dishes depends on the temperature of the oven. It is recommended to place the oven at 160 degrees C for one to two hours, or 180 degrees C for 20 minutes.

Where is the proper place to dispose of your petri plates after you are done examining them?

The petri dishes must be collected from the students for proper disposal. They should either be autoclaved in a disposable autoclave bag (see supplies list) or they can be opened and flooded with 10% bleach for about 30 minutes before they are disposed of in the regular trash in a plastic garbage bag.

How would you properly dispose of a petri dish containing a fungal culture?

How do you dispose of a Petri Dish containing a fungal culture? Dispose in the biohazard waste container.

What Cannot be killed by autoclave?

The short answer: no. Autoclaves are capable of killing all types of microorganisms such as bacteria, viruses, and even spores, which are known to survive at high temperatures and can only be killed at temperatures around 130°C.

When do you need to use a sterilized Petri dish?

If your experiment requires a 100 percent pure culture and you’re using plastic Petri dishes, then its best use new dishes sterilized from the manufacturer. Do not reuse plastic Petri dishes if your experiment involves the use of live pathogens. The risk of cross-contamination is too great.

When to remove glass petri dishes from oven?

Set the timer for two hours. After two hours, turn the oven off and allow the oven to cool prior to removing the glass Petri dishes. Remove the Petri dishes from the oven using sterile lab tongs. Do not allow your fingers or any unsterile material to touch the sterilized Petri dishes.

How to dispose of plastic agar plates and Petri dish?

Dispose the plastic chunk and pour water into a biohazardous liquid receptacle or a drain where it can be safely disposed and eventually processed. Safe, easy and no mess! As you can see, petri dish sterilization can be done safely and easily with little mess, drastically reducing the risk of contamination.

Where can you find a petri dish in a science lab?

Petri dishes are a common item found in both professional and educational science labs. Unfortunately, budget restrictions force companies and educational establishments, such as high school and college biology labs, to reuse the Petri dishes.